Pierre Guerin Technologies EVO 50-60L Fermenter

This listing is for a Pierre Guerin Technologies EVO 50-60L Fermenter.

The unit is in full working condition and is ready for immediate release.

Please see spreadsheet attached at the bottom of listing for all parts included with this unit.

The Pierre Guerin EVO Series fermenters and bioreactors are precision-engineered systems designed for advanced bioprocess research, development, and small-scale production. These mobile, Sterilize-In-Place (SIP) and Clean-In-Place (CIP) units are optimal for applications ranging from educational settings and R&D to pilot-scale productions within GLP or GMP environments. They are versatile and suitable for various culture types, including yeast, bacteria, fungi, or cell cultures, operating in batch, fed-batch, continuous, or perfusion modes (with appropriate accessories).

Key Features:

Vessel Design:

• Capacity & Configuration: Working volume of 50-60L with a total volume of 75L.
• Material & Surface Finish: High-grade 316L stainless steel, with an internal surface finish of Ra ≤ 0.8 µm and pipework Ra ≤ 1.6 µm, ensuring sterility and cleanability.
• Sterilization & Temperature Control: Double jacket for automatic media sterilization and precise culture temperature regulation.
• Operating Pressure: Maximum vessel pressure of 4 barg, jacket pressure up to 6 barg.
• Headplate Design: Removable flat headplate for easy access and maintenance.

Agitation System:

• Drive Type: Available in mechanical coupling with a single seal or magnetic coupling with a synchronous drive for reduced contamination risk.
• Impeller Configuration: Standard setup includes one HTPG4 impeller, one Rushton impeller, and one baffle, providing optimal mixing for various culture types.
• Speed Range: 30 to 1200 RPM with precision control.
• Torque Monitoring: Integrated system for monitoring agitation performance.

Instrumentation & Control:

• Measurement & Control:
• Temperature: PID-controlled temperature regulation.
• Agitation Speed: Precise real-time adjustment.
• pH: Online monitoring and automated control.
• pO₂ (Dissolved Oxygen): Optical probe-based real-time monitoring and control.
• Foam Level: Automatic detection and antifoam control.
• Weight Measurement: Integrated load cell system.
• Total or Viable Cell Density Measurement: Optional high-accuracy sensors available.
• Neptune Control System:
• Graphical Interface: Intuitive touchscreen display.
• GMP Compliance: 21 CFR PART 11 & GAMP 5 validation-ready.
• Advanced Process Control: Includes profile management, real-time calculations, and automation strategies.
• Remote Monitoring & Data Logging: Supports Ethernet™ network integration for remote supervision and maintenance.

Gas & Fluid Management:

• Gas Control: Automatic control of air, oxygen, nitrogen, and CO₂ sparging.
• Peristaltic & Precision Dosing Pumps: Integrated system for nutrient and pH adjustments.
• Media Addition & Sampling: Dedicated ports for sterile sampling and automated feed control.

Cleaning & Sterilization:

• SIP (Sterilize-In-Place): Automated sterilization cycle.
• CIP (Clean-In-Place): Fully integrated system for effortless cleaning between batches.

Specifications:

• Nominal Working Volume: 50-60 liters
• Minimum Working Volume: 35 liters
• Total Volume: 75 liters
• Agitation Speed Range: 30-1200 RPM
• Material: 316L stainless steel with Ra ≤ 0.8 µm finish
• Pressure Ratings: Maximum operating pressure 2 barg for vessel and 2.5 barg for jacket.
• Sterilization: SIP & CIP capability
• Control System: Neptune SCADA-based, remote monitoring ready
• Gas Control: Automated sparging system for O₂, N₂, CO₂, and air
• Pumps: Integrated peristaltic dosing & feed pumps
• Process Modes: Batch, Fed-Batch, Continuous, Perfusion

Parts List:

https://docs.google.com/spreadsheets/d/e/2PACX-1vTcgag74SsDZA3GFLiVGwX0gaGkhdSnIhGggiXlBQcxkRlRcP_vJxJ2gaCPhmyV2Mc76OwB6FHQTvYO/pubhtml

Brochure:

https://www.pierreguerin.com/sites/g/files/tkmtob276/files/2023-10/Evo_Data%20Sheet_UK.pdf